Given the larger number of total BrdU-ir cells in birds injected on E8 or E10, the doublets were also more frequent in absolute numbers in birds injected at these ages but they were still clearly present in the E12 and E14 groups. The counting field was placed at the edge of the third ventricle just ventral to the posterior commissure.
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This PCNA expression suggests that some of the cells labeled by BrdU during embryonic life are cycling at low frequency.
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